The position and shape of the excretory hole can distinguish male and female: The male Florida box turtle is located near the tail, and the hole is elongated. The female Florida box turtle is located close to the plastron and has a rounded hole.

You can also see the thickness of the tail, the male Florida box turtle is thicker, Female Florida box turtles are slender and shorter. In addition, you can also see whether the plastron is concave, the male Florida box turtle has obvious depression, and the female Florida box turtle is flat.