Infusions for pets are often administered for severe blood loss, severe anemia, to increase blood solids, and to improve pet stamina. Although blood types also exist in pets, blood types are often not considered in the first blood transfusion of pets.

(1) Selection of blood donor animals

Choose those with strong physique and healthy (clinical and laboratory tests) and not too obese Same kind of pet. The appropriate weight is about 20kg for dogs and 2kg for cats.

(2) Blood group and blood compatibility test

Blood group and cross-match test is applicable to the same pet for repeated blood transfusion. Dogs have seven blood factors, of which blood group A is the most important. Infusion of A-positive blood to A-negative dogs can induce the production of anti-A antibodies. The antibody has strong hemolytic activity in vitro and in vivo. If male dogs stimulated by A-positive blood are mated with A-positive male dogs, the pups born to them will develop neonatal hemolysis after breastfeeding. Among the dogs, 37% of the dogs were A-negative and 63% were A-positive. If blood was transfused blindly, 25% of the dogs could produce anti-A antibodies. In the second blood transfusion, 15% of the dogs who blindly choose blood supply are unsuitable blood transfusions, and it is very dangerous to transfuse A-positive blood 2-3 times to A-negative dogs. At present, it is impossible to determine the blood type of a dog by an accurate method, so only the blood compatibility test is used to determine whether the two are suitable. The specific methods are as follows:

①Collect the anticoagulated blood from the donor and the recipient, and collect serum and red blood cells (anticoagulated and non-anticoagulated) respectively.

②Wash the red blood cells 3 times and make a 5%-10% red blood cell suspension with plasma.

③ Add 2 drops of serum from recipient individuals and red blood cell suspension from donor individuals into a thin test tube (7mm in diameter) and mix. Then add 2 drops of serum from the donor individual and 2 drops of red blood cells from the recipient individual into another test tube and mix.

④Leave the test tube at room temperature for 30 minutes, then centrifuge at 1000rpm for 1 minute.

⑤Any combination of obvious hemolysis or coagulation indicates that the blood of the donor and recipient does not meet the requirements.

⑥It is simpler to mix the donor and recipient serum and plasma on the glass slide, and the hemolysis and coagulation appear to be inconsistent with blood.

⑦The three-drop test method can also be used: take a clean glass slide, drop a drop of normal saline in the center, and drop a drop of donor blood and recipient blood on both sides of the normal saline drop. Mix the three drops evenly with a glass rod, let stand for 3-5 minutes, observe the state of the mixture, if the mixture is evenly distributed, blood can be provided. If the mixture appears granular precipitation, it means that the two blood types are incompatible and cannot be used as blood donor animals.